Basic mutant information
Mutant name	arf2-8 nph4-1 arf19-4
Mutant/Transgenic	mutant
Ecotype	Col-0
Mutagenesis type	cross
(Semi-)Dominant/Recessive	recessive
Description	Male sterile. Although mutations in NPH4/ARF7 and ARF19 did not affect senescence of rosette leaves or floral organs on their own, they enhanced the effects of the arf2-8 mutation. Eight days after detachment, arf2-8 leaves retained 49% of their chlorophyll, whereas arf2-8 nph4-1 arf19-4 leaves retained 65%.
References	1: Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana. Development 2005 Oct;132(20):4563-74
Mutated genes
Mutated Gene 1 Locus name AT5G62000 Alias ARF2/HSS/ORE14 Organism Arabidopsis thaliana Description Encodes an auxin response factor. Mutants have many defects including enlarged rosette leaves, reduced fertility, later senescence, hypocotyl elongation defects, enlarged seeds and enlarged cotyledons. May not mediate auxin effects. Increase in seed size due to increased cell proliferation.
Mutated Gene 2 Locus name AT1G19220 Alias ARF19 Organism Arabidopsis thaliana Description Encodes an auxin response factor 19.
Phenotype information
Darkness induced senescence Chlorophyll content high Marker gene expression no